“Spatial SILAC—Developing Isotopic Zip Codes for Drug Development in 3D Cell Cultures”
Amanda Hummon, PhD
Professor of Chemistry and Biochemistry
The Ohio State University
Zoom Webinar Details
Webinar URL: https://stanford.zoom.us/s/97546627376
Dial US: +1 650 724 9799 or +1 833 302 1536
Webinar ID: 975 4662 7376
Three-dimensional cell cultures are attractive models for biological research. They combine the flexibility of cell culture with some of the spatial and molecular complexity of tissue. For example, colon cancer cell lines form spheroids, in vitro mimics of poorly vascularized tumors. The spheroids are composed of a central necrotic core, a middle quiescent layer and an outer proliferative layer of cells, similar to a rapidly growing colon tumor. Our laboratory has characterized the distribution of endogenous proteins via MALDI imaging mass spectrometry in colon spheroids and determined that the molecular gradients correlate with the pathophysiological changes in the structure. We have developed Spatial SILAC, a method to selectively label the cells in the distinct chemical microenvironments in the spheroids and assess the proteomic changes in response to drug treatment. In this presentation, the spatially-localized proteomic changes in response to the multiple kinase inhibitor Regorafenib will be described. In brief, the cellular response to Regorafenib treatment is significantly affected by the location of the cells and the chemical microenvironment.
Amanda Hummon earned her A.B. in chemistry at Cornell University and her Ph.D. in analytical chemistry at the University of Illinois, in the laboratory of Prof. Jonathan V. Sweedler. She completed her postdoctoral work at the National Cancer Institute. She is currently a Professor in the Department of Chemistry and Biochemistry and the Comprehensive Cancer Center at The Ohio State University. Her laboratory develops mass spectrometric methods to explore cancer tissues, cell cultures, and organoids. She has been recognized with an NSF CAREER award, the American Chemical Society Rising Star Award and the Presidential Early Career Award for Scientists and Engineers (PECASE). She recently completed a Fulbright Scholar Award in the Laboratory of Ron Heeren in Maastricht, the Netherlands.
Hosted by: Utkan Demirci, Ph.D.
Sponsored by: The Canary Center & the Department of Radiology
Stanford University – School of Medicine